The phragmoplast is a special apparatus that functions in establishing a cell plate in dividing plant cells. It is constituted of two bundles of anti-parallel microtubules, microfilaments (MFs) and membranous elements between them. The figure shows the phragmoplast MFs of a BY-2 suspension cell stably expressing the MFs reporter GFP-fABD2 and the forming cell plate labeled by FM4-64. The green fluorescence shows the microfilaments which mainly form the phragmoplast microfilaments composed of two layers of parallel microfilament arrays that interdigitate in the interzone. The red fluorescence represents the FM4-64 labeled vesicles, which concentrate in the interzone and are building the cell plate. It was long thought that the MFs of the phragmoplast were not interdigitated but separate. Our results clearly showed the interdigitated MFs (see the article by ZHANG Yan et al. on page 2051).
· CNKI · Wamfangdata