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advanced oxidation protein products |
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podocyte |
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nephrin |
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podocin |
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NADPH oxidase |
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Advanced oxidation protein products decrease expression of nephrin and podocin in podocytes via ROS-dependent activation of p38 MAPK
YANG Li†,LIANG Min†,ZHOU QiuGen,XIE Di,LOU AiJu,ZHANG Xun&HOU FanFan*
Key Laboratory for organ failure research, Division of Nephrology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Accumulation of plasma advanced oxidation protein products (AOPPs) promotes progression of proteinuria and glomerulosclerosis. To investigate the molecular basis of AOPPs-induced proteinuria, normal Sprague-Dawley rats were treated with AOPPs-modified rat serum albumin. The expression of glomerular podocyte slit diaphragm (PSD)-associated proteins, nephrin and podocin, was significantly decreased coincident with the onset of albuminuria in rats treated with AOPPs. Chronic inhibition of NADPH oxidase by apocynin prevented down-regulation of nephrin and podocin and decreased albuminuria in AOPPs-challenged rats. This suggested that accumulation of AOPPs promotes proteinuria, possibly via down-regulating the expression of PSD-associated proteins.
This study was supported by the National Natural Science Foundation of China (Grant Nos. 30830056 and 30830056), the National Basic Research Program of China (Grant No. 2006CB503904) to Dr. HOU FanFan, the National Natural Science Foundation of China （Grant No. 30971382）and the Natural Science Foundation of Guangdong Province (Grant No. 06024402) to Dr. LIANG Min. We thank Professor Peter Mundel for providing murine podocytes.